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Eric Bühler, Eric Bühler Department of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim , 70593 Stuttgart , Germany Find other works by this author at: Oxford Academic Elisa Fahrbach Department of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim , 70593 Stuttgart , Germany Find other works by this author at: Oxford Academic Andrew Schaller Department of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim , 70593 Stuttgart , Germany Find other works by this author at: Oxford Academic Nils Stührwohldt Department of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim , 70593 Stuttgart , Germany Correspondence: Nils Stührwohldt,nils.stuehrwohldt@uni-hohenheim.de Find other works by this author at: Oxford Academic
https://orcid.org/0009-0003-0703-3817Plant Physiology, child316,https://doi.org/10.1093/plphys/kiad316
Posted by:
May 31, 2023
Article history
Received:
March 20, 2023
Download Review:
April 21, 2023
Editorial decision:
April 24, 2023
Accepted:
May 30, 2023
Posted by:
May 31, 2023
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Eric Bühlerand othersThe CLEL6 sulfo-peptide inhibits anthocyanin biosynthesis inArabidopsis thaliana,Plant Physiology, 2023;, kiad316,https://doi.org/10.1093/plphys/kiad316
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Abstract
Post-translationally modified peptides are now recognized as important regulators of plant stress responses. Here, we identified the small sulfate peptide CLE-LIKE6 (CLEL6) as a negative regulator of anthocyanin biosynthesis in etiolated and mildly stressed Arabidopsis (Arabidopsis thaliana) seedlings. CLEL6 function depends on proteolytic processing of the CLEL6 precursor by subtilisin-like serine proteinase 6.1 (SBT6.1) and on tyrosine sulfation by tyrosylprotein sulfotransferase (TPST). Loss-of-function mutants of eachsbt6.1thetpstshowed significantly higher anthocyanin accumulation than the wild type during light stress. Its anthocyanin hyperaccumulation phenotypesbt6.1andtpstsuppressed by applying mature CLEL6. Overexpression and exogenous application of CLEL6 inhibited the expression of anthocyanin biosynthesis genes in aetiology and mildly stressed plants, confirming the role of CLEL6 as an inhibitor of anthocyanin biosynthesis. Small post-translationally modified peptides are sensed by leucine-rich receptors such as kinases. Using a ROOT MERISTEM GROWTH FACTOR 1 INSENSITIVE (RGI) receptor quintuple mutant, we demonstrated the essential function of the RGI receptor family in CLEL6 signaling. Our data show that overexpression or knockdown of CLEL6 inhibits anthocyanin biosynthesis through RGI receptors. We propose that CLEL6 inhibits anthocyanin biosynthesis in plants causally and that anthocyanin biosynthesis is restored whenCLEL6Expression is down-regulated upon exposure to light. Hyperaccumulation of anthocyanins in light stressestpstandsbt6.1Mutant seedlings suggest that CLEL6, or related sulfopeptides, continue to act as negative regulators to limit pigment accumulation in light.
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